Estimation of Marbling Score by Ultrasonography and Visual Methods of Male Bali Beef Based on Muscle Location and Storage Time

Muh. Muazdzamzam Lil Abrori (1), Bulkaini Bulkaini (2), Hasma Hasma (3), Djoko Kisworo (4), Fahrullah Fahrullah (5), Novizar Nazir (6), Muhammad Ashari (7)
(1) Animal Science Resource Management Study Program, Faculty of Animal Science, University of Mataram, Mataram, West Nusa Tenggara, Indonesia
(2) Animal Science Resource Management Study Program, Faculty of Animal Science, University of Mataram, Mataram, West Nusa Tenggara, Indonesia
(3) Animal Science Resource Management Study Program, Faculty of Animal Science, University of Mataram, Mataram, West Nusa Tenggara, Indonesia
(4) Animal Science Resource Management Study Program, Faculty of Animal Science, University of Mataram, Mataram, West Nusa Tenggara, Indonesia
(5) Animal Science Resource Management Study Program, Faculty of Animal Science, University of Mataram, Mataram, West Nusa Tenggara, Indonesia
(6) Faculty of Agricultural Technology Unand, Limau Manis Campus, Kota Padang, Sumatra Barat 25175, Indonesia.
(7) Animal Science Resource Management Study Program, Faculty of Animal Science, University of Mataram, Mataram, West Nusa Tenggara, Indonesia
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Abrori, M. M. L., Bulkaini, B., Hasma, H., Kisworo, D., Fahrullah, F., Nazir, N., & Muhammad Ashari. (2025). Estimation of Marbling Score by Ultrasonography and Visual Methods of Male Bali Beef Based on Muscle Location and Storage Time. AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment), 9(3), 193–199. https://doi.org/10.29165/ajarcde.v9i3.807

Marbling refers to white fat granules within muscle fibres that enhance meat tenderness and flavour. This study aimed to determine the marbling score of male Bali beef using two methods: ultrasonography and visual assessment based on muscle location and storage duration. Five intensively raised male Bali cattle (average weight 227 kg) were examined. Visual assessment used 0.5 kg samples from sirloin, rib, and round muscles, analysed with ImageJ and Minitab 17. Results showed that both muscle location and storage duration significantly affected (P < 0.01) the marbling score. The ultrasonography method yielded marbling scores of 1.06–1.44, while the visual method produced 1.10–1.50. The ultrasonography method proved reliable for predicting marbling without slaughter, with higher marbling observed in passive muscles (sirloin) than in active muscles (rib and round).


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[1] Jakaria, J., Khasanah, H., Priyanto, R., Baihaqi, M., Ulum, M.F. (2017).Prediction of quality in Bali catle using ultrasound imaging. Journal of the Indonesian Tropical Animal Agriculture, 42(2):59-65. https://doi.org/10.14710/jitaa.42.2.59-65.

[2] Bulkaini, B. (2022). Production Performance and Carcass Quality of Male Bali Cattle by Feeding Fermented Pineapple Peel. Asian Journal of Applied Research for Community Development and Empowerment. 6(2): 29-34. https://doi.org/10.29165/ajarcde.v6i2.96.

[3] Aredo, V., Velásquez, L., Siche, R. (2017). Prediction of beef marbling using Hyperspectral Imaging (HSI) and Partial Least Squares Regression (PLSR). Scientia Agropecuaria 8(2): 169 – 174 . DOI: 10.17268/sci.agropecu.2017.02.09.

[4] Shiddieqy, M.I., Pratiwi, N., Soewandi, B.D.P. (2009). Utilization of Molecular Marker to Improve Cattle Carcass Quality in Indonesia, WARTAZOA, 29(4): 193-204 DOI: http://dx.doi.org/10.14334/wartazoa.v29i4.

[5] Badan Standarisasi Nasional (2008). SNI 3932:2008 tentang Mutu Karkas dan Daging Sapi. Jakarta: Badan Standarisasi Nasional. http://www.suluhtani.com/2014/11/lemak-pada-daging-sapi-ini-jenisnya.html.

[6] Diana, C., Dihansih, E., Kardaya, D.(2018). Kualitas Fisik dan Kimiawi Daging Sapi Beku Pada Berbagai Metode Thawing. Jurnal Petanian, 9(1): 51-60 https//ojs.unida.ac.id/issue/view/236.

[7] Supriatin, S., & Prambudi, H.(2020). Kajian Kualitas Kimia Daging Sapi Tenderloin dan Sirloin di RPH Tradisional di Kabupaten Cirebon. Jurnal Health Sains, 1(3), 169-177. https://doi.org/10.46799/jhs.v1i3.25.

[8] Trantono, Y.(2024). Bangsa-bangsa sapi potong. https://yuari.wordpress.com/about/6213_1090223899261_1336400769_30235673_4233792_n/.

[9] Stewart, S.M., Gardner, G.E., McGilchrist, P., Pethick, D.W., Polkinghorne, R.(2021). Thompson, J.M., Tarr, G. Prediction of consumer palatability in beef using visually marbling scores and chemical intramuscular fat percentage. Meat Science, 181: 108322. https://doi.org/10.1016/j.meatsci.2020.108322.

[10] Veni, R.V.S., Isdoni, I,. Tobing, A.S.P.L., Ulum, M.F.(2024) Estimasi kualitas karkas hidup pada kerbau rawa dan kerbau sungai dengan USGpada berbagai umur. Jurnal Ilmiah Peternakan Halu Oleo, 6(1): 13-23. https://ojs.uho.ac.id/index.php/jipho/issue/view/2630.

[11] Rueden, C.T., Schindelin, J., Hiner, M.C., DeZonia, B.E., Walter, A.E., Arena, E.T. Eliceiri, K.W.(2017). ImageJ2: ImageJ for the next generation of scientific image data. BMC bioinformatics, 18: 1-26. DOI 10.1186/s12859-017-1934-z.

[12] Ulum, M.F., Prisetiadi, A., Pamungkas, F.A., Jakaria, J.(2024). Estimasi Skor Marbling dan Lemak Intramuskular Sapi Bali dan Sapi Sumba Ongole Menggunakan Program ImageJ pada Citra Ultrasonografi. Wahana Peternaka, 8(2): 169-176. DOI: https://jurnal.utb.ac.id/index.php/jwputb/article/view/1448.

[13] Gunawan, L.(2013). Analisa Perbandingan Kualitas Fisik Daging Sapi Impor dan Daging Sapi Lokal. Jurnal Hospitality dan Manajemen Jasa, 1(1) :146-166. https://www.neliti.com/publications/80764/analisa-perbandingan-kualitas-fisik-daging-sapi-impor-dan-daging-sapi-lokal

[14] Fatah, A.(2011). Kadar Kolesterol Daging Kerbau Dengan Metode Pemasakan yang Berbeda. [Skripi]. Program Studi Peternakan Fakultas Pertanian dan Peternakan Universitas Islam Negeri Sultan Syarif Kasim Riau Pekanbaru. https://repository.uin-suska.ac.id/2218/1/2011_201128.pdf.

[15] Savell, J.W., Branson, R.E., Cross, H.R., Stiffler, D.M., Wise, J.W., Griffin, D.B., Smith, G.C. (1987). National consumer retail beef study: palatability evaluations of beef loin steaks that differed in marbling. Journal of Food Science, 52, 517–519. Doi: 10.1111/j.1365-2621.1987.tb06664.x.

[16] Jung, S., Nam, K. C., Lee, K. H., Kim, J. J., Jo, C.(2013). Meat quality traits of Longissimus dorsi muscle from carcasses of Hanwoo steers at different yield grades. Food Science of Animal Resources, 33(3): 305-316. DOI http://dx.do.org/10.5851/kosfa.2013.33.3.305.

[17] Killinger, K.M., Calkins, C.R., Umberger, W.J., Feuz, D.M., Eskridge, K.M.(2004). A comparison of consumer sensory acceptance and value of domestic beef steaks and steaks from a branded, Argentine beef program. Journal of Animal Science, 82, 3302–3307. https://doi.org/10.2527/2004.82113302x.

[18] Morales, R., Aguiar, A.P,S., Subiabre, I., Realini, C.E.(2013). Beef acceptability and consumer expectations associated with production systems and marbling. Food Quality and Preference 29, 166–173. Doi:10.1016/ j.foodqual.2013.02.006.

[19] Corbin, C.H., O’Quinn, T.G., Garmyn, A.J., Legako, J.F., Hunt, M.R., Dinh, T.T.N., Rathmann, R.J., Brooks, J.C., Miller, M.F.(2015). Sensory evaluation oftender beefs triploinsteak sofvarying marbling levels and quality treatments. Meat Science, 100: 24 31. Doi:10.1016/j.meatsci.2014.09.009.

[20] Hidayat, M.A., Kuswati, K., Susilawati, T. (2016). Pengaruh lama istirahat terhadap karakteristik karkas dan kualitas fisik daging sapi Brahman Cross Steer. Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science), 25(2), 71-79. https://doi.org/10.21776/ub.jiip.2015.025.02.09.

[21] Rahayu, S. (2009). Sifat Fisik Daging Sapi, Kerbau dan Domba pada Lama Postmortem yang Berbeda (Physical Characteristics of Beef, Buffalo and Lamb Meat on Different Postmortem Periods). Buletin Peternakan, 33(3): 183-189. https://doi.org/10.21059/buletinpeternak.v33i3.115.

[22] Mustofa, M., Farouk, F., David, I., Jenkison, J., Zhang, R., Carolina, E., Realini, R.(2024). Mincing words: Valorisation of mince beef through nutrient/functional formulation. Project code: P.PSH.1388. PUBLISHED BY Meat & Livestock Australia Limited, Locked Bag 1961, North Sydney NSW2059. https://www.mla.com.au/nutritional-valorisation-of-mince-beef_27june2024-3.pdf.

[23] Chicco, D., Warrens, M.J., Jurman, G. (2021). The coefficient of determination R-squared is more informative than SMAPE, MAE, MAPE, MSE and RMSE in regression analysis evaluation. PeerJ Computer Science, 7:e623. https://doi.org/10.7717/peerj-cs.623.

[24] Lambe, N.R., Navajas, E.A., Simm, G., Bunger, L.(2010). Prediction of intramuscular fat in beef using ultrasound and carcass measurements. Meat Science, 85(1): 1–7. https://doi.org/10.1016/j.meatsci.2009.12.002.

[25] Luz, P.A.C.D., Jorge, A.M., Francisco, C.D.L., Mello, J.L.M.D., Santos, C.T., Andrighetto, C. (2017). Chemical physical characteristics of buffalo (Bubalus bubalis) meat subjected to different aging times. Acta Scientiarum. Animal Sciences, 39: 419-428. https://doi.org/10.4025/actascianimsci.v39i4.36799.

[26] Bulkaini, B., Kiswolro, D., Fahrullah, F., Sumadi, I. K., & Nazir, N. (2025). Characteristics of Carcass and Marbling Score of Male Bali Cattle Using Herbal Supplement of Molasses Block Based on Fermented Pineapple Peel. AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment), 9(2), 59-65. http://ajarcde-safe-network.org.

[27] Irshad, A., Kandeepan, G., Kumar, S., Kumar, A.A., Vishnuraj, M.R., Shukla, V.(2012). Factors influencing carcass composition of livestock: a review. Journal of Animal and Poultry Advances. 3(5): 177-186. http://dx.doi.org/10.5455/japa.20130531093231.

[28] Bulkaini, B., Dahlanudin, D., Ariana, T., Kisworo, D., Maskur, M., Mastur, M.(2022). Marbling Score, Cholesterol, And Physical–Chemical Content Of Male Bali Beef Fed Fermented Pineapple Peel. Journal Of Advanced Veterinary And Animal Research, 9(3): 419-431 http://doi.org/10.5455/javar.2022.i610.

[29] Suryanto, E., Bulkaini, B., Soeparno, S., Karda, I.W.(2017). Kualitas karkas, marbling, kolesterol daging dan komponen non karkas sapi bali yang diberi pakan kulit buah kakao fermentasi. Buletin Peternakan, 41(1): 72-78. https://doi.org/10.21059/buletinpeternak.v41i1.12757.

[30] Soeparno. (2015). Ilmu dan Tenologi Daging. Edisi Revisi Cetakan ke enam. Gajah Mada University Press, Yogyakarta, Indonesia.

[31] Fiore, E., Fabbri, G., Gallo, L., Morgante, M., Muraro, M., Boso, M., Gianesella, M.(2020). Application of texture analysis ultrasound images of b-mode for the quantification and prediction of intramuscular fat in living beef cattle: A methodological study. Research in veterinary science. 131:254-8. https://doi.org/10.1016/j.rvsc.2020.04.020.

[32] Lee, Y., Oh, D., Lee, J., La, B., Yeo, Y.(2023). Novel single nucleotide polymorphisms of bovine SREBP1 gene is association with fatty acid composition and marbling score in commercial Korean cattle (Hanwoo). Mol. Biol. Rep. 1: 247–254. https://doi.org/10.1007/s11033-012-2055-4.

[33] Shin, S.C., Heo, J.P., Chung, E.R.(2012). Genetic variants of the FABP4 gene are associated with marbling scores and meat quality grades in Hanwoo (Korean cattle). Molecular Biology Report. 5:5323–5330. https://doi.org/10.1007/s11033-011-1331-z.

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