DOI : https://doi.org/10.29165/ajarcde.v9i3.762

Effect of Trigona Bee Propolis Extract in Duck Egg Yolk Tris Diluent on the Quality of Boer Goat Spermatozoa at 5°C

S Adawiah (1), Lalu Zaenuri (2), IWL Sumadiasa (3)
(1) Graduate Student, Faculty of Animal Husbandry, Mataram University, Majapahit Street No. 62, Mataram 83125, West Nusa Tenggara, Indonesia
(2) Animal Reproduction Laboratory, Faculty of Animal Husbandry, Mataram University, Majapahit Street No. 62, Mataram 83125, West Nusa Tenggara, Indonesia
(3) Animal Reproduction Laboratory, Faculty of Animal Husbandry, Mataram University, Majapahit Street No. 62, Mataram 83125, West Nusa Tenggara, Indonesia
Fulltext View | Download
How to cite (AJARCDE) :
Adawiah, S., Zaenuri, L., & Sumadiasa, I. (2025). Effect of Trigona Bee Propolis Extract in Duck Egg Yolk Tris Diluent on the Quality of Boer Goat Spermatozoa at 5°C. AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment), 9(3), 61–65. https://doi.org/10.29165/ajarcde.v9i3.762
Citation Format :

This study aimed to determine the quality of Boer goat spermatozoa when stored at 5°C in a duck egg yolk-tris diluent supplemented with propolis extract. The material used was fresh semen collected from ±2-year-old Boer goats using an artificial vagina. This research employed a laboratory experimental method with a Completely Randomized Design (CRD), consisting of four propolis extract treatments: 0% (T0), 0.25% (T1), 0.5% (T2), and 1% (T3), with five replications. Observed variables included motility, viability, and abnormality. Observations were conducted every day at the same time. Data were analysed using Analysis of Variance (ANOVA) and subsequently tested with Duncan's multiple range test. The research results indicate that the best average sperm motility was found in T1, at 54.40±11.14%, followed by T0 at 41.00±12.36%, T2 at 40.40±11.76%, and T3 at 39.60±2.88%. The highest sperm viability was observed in T1, 58.40±6.06%, followed by T3, 49.20±4.65%, T2, 45.60±9.04%, and T0, 44.00±6.85%. The lowest sperm abnormality was found in T1, 4.60±1.87%, followed by T2, 6.00±1.87%, T3, 5.80±4.81%, and T0, 5.00±2.44%. In conclusion, among the four different diluents, the addition of 0.25% propolis extract in Tris-diluted egg yolk diluent showed the highest ability to maintain the quality of Boer goat spermatozoa for three days at a storage temperature of 5°C. Further studies are needed on the effects of different concentrations of propolis extract, various egg yolks, and different temperatures


Contribution to Sustainable Development Goals (SDGs)
SDG 2 (Zero Hunger)
SDG 3 (Good Health and Well-being)
SDG 12 (Responsible Consumption and Production)

[1] Anwar, P., & Rodiallah M. 2015. Biotekhnoogi Reproduksi. Universitas Islam Negeri Sultan Syarif Kasim Riau.

[2] Awang, M. T. L., Kaka, A. & Pati, D. U. 2022. Kualitas spermatozoa sapi sumba ongole dalam pengencer tris kuning telur yang disimpan pada suhu ruang. Jurnal Peternakan Sabana. DOI: https://doi.org/10.58300/jps.v1i1.224

[3] Bankova, V., Popova, M & Trusheva B. 2014. Propolis volatile compounds?: chemical diversity and biological activity?: a review, 8: 28–35. http://journal.chemistrycentral.com/content/8/1/28

[4] Bintara S., 2011. Rasio spermatozoa X : Y dan kualitas semen pada kambing Kacang dan kambing Peranakan Etawa. Jurnal Sains Peternakan., 9 (2): 65-71.

[5] Ducha N., Susilawati, T., Aulanni’am, & Wahjuningsih S. 2013. Motilitas dan viabilitas spermatozoa sapi limousin selama penyimpanan pada refrigerator dalam pengencer CEP-2 dengan suplementasi kuning telur. Jurnal Kedokteran Hewan, 7(1): 5-8. http://dx.doi.org/10.21157/j.ked.hewan.v7i1.555

[6] Evans G., & Maxwell, W.M.C. 1987. Salamon’s Artificial insemination of Sheep and Goats. Butterworths, Sydney, NSW, Australia: University Press. 22-141.

[7] Hikmawan, S.W., Ciptadi, G., &. Wahyuningsih, S. 2016. Kualitas spermatozoa swim up kambing peranakan etawah hasil pembekuan menggunakan metode vitrifikasi dengan persentase gliserol yang berbeda. Jurnal Ternak, 4(2): 45–52. https://repository.ub.ac.id/id/eprint/137888

[8] Kartasudjana, R. 2001. Teknologi Inseminasi Buatan Pada Ternak. Jakarta

[9] Kusumawati E.D., Leondro H., Krisnaningsih A.T.N., Susilawati, T., Isnaini, N.& Widhad, R. 2016. Pengaruh Suhu dan Lama Simpan Semen Segar Terhadap Motilitas dan Abnormalitas Spermatozoa Kambing Peranakan Etawa (PE). Prosiding Seminar Nasional Hasil Penelitian, 199-208

[10] Manehat, F.X. 2021. Motilitas, Viabilitas, Abnormalitas Spermatozoa Dan PH Semen Sapi Bali Dalam Pengencer Dari Air Tebu-Kuning Telur Yang Disimpan Dalam Waktu Yang Berbeda (Doctoral Dissertation, Universitas Timor).

[11] Munazaroh, A.M., Wahjuningsih S., & Ciptadi, G. 2013. Uji Kualitas Spermatozoa Kambing Boer Hasil Pembekuan Menggunakan Mr. Frosty® pada Tingkat Pengenceran Andromed® Berbeda. TERNAK TROPIKA Journal of Tropical Animal Production, 14(2): 63-71. https://repository.ub.ac.id/id/eprint/137060

[12] Nilna. 2012. Standar Operasional Pekerjaan Prosesing Semen. Dinas Peternakan Sumatera Barat.

[13] Novita, R., Karyono, T & Rasminah, R. 2019. Kualitas Semen Sapi Brahman pada PersentaseTris Kuning Telur yang Berbeda. Jurnal Sain Peternakan Indonesia, 14(4): 351–358. https://ejournal.unib.ac.id/jspi/article/view/6939

[14] Nugroho, W.S., & Saleh, M. 2016. Kualitas Spermatozoa dan Hubungannya dengan Penanganan Pascaproduksi. Yogyakarta: Gadjah Mada University Press.

[15] Nurhayati, S.Z., Solihat, N., Setiawan. R. 2025. Pengaruh Kadar Resiquimod (R848). The Effect of Resiquimod (R848) Levels on The Morphometrics Of Spermatozoa X And Y In Etawah Crossbreed Goats Semen. Jurnal Produksi Ternak Terapan. 06(01): 23-26. https://doi.org/10.24198/jptt.v6i1.55886

[16] Rahayu, T.I., Marcelina, Y., Sinaga R., Perdhana, F.F., Zuhdia, L.D. 2024. Optimasi Proses Ekstraksi Propolis Dari Limbah Perasan Madu Trigona Dengan Penggunaan Pelarut Air. Pro Food (Jurnal Ilmu dan https://doi.org/10.56338/jks.v7i6.5375 Ekstrak Air Propolis Trigona spp Asal Lombok. Jurnal Kolaboratif Sains, 7(6): 1954–1962.

[17] Steel, R.G.D., Torrie, J.H. 1993. Prinsip dan prosedur Statistika, Suatu Pendekatan Biometrik. Terjemahan Sumantri B. Jakarta. Gramedia Pustaka Utama.

[18] Susilawati, T. 2013. Sementology. UB Press, Malang.

[19] Suyadi, Rachmawati, A.& Iswanto, N. 2012. Pengaruh A-Tocopherol yang Berbeda dalam Pengencer Dasar Tris Ainomethane Kuning Telur Terhadap Kualitas Semen Kambing Boer yang Disimpan pada Suhu 5 C. Jurnal Ilmu-Ilmu Peternakan, 22(3): 1-8.

[20] Yimer, N., Yilma, T. & Tadesse, F. 2015. Semen quality characteristics of Boer goats under natural mating condition in Ethiopia. African Journal of Biotechnology, 14(18): 1544–1548.

[21] Zaenuri, L.A. 2018. Inseminasi Buatan Pada Ternk Kambing. Mataram University Press. Mataram.

[22] Zaenuri, L.A., Rodiah, R. 2025. Impact of fig-enriched tris-albumin extender on the quality of boer buck spermatozoa. Adv. Anim. Vet. Sci., 13(1): 26-35. https://dx.doi.org/10.17582/journal.aavs/2025/13.1.26.35

[23] Zaenuri, L.A., Rodiah, R., Lukman, H.Y., Dradjat, A.S., Yuliani, E. 2023. Spermatozoa quality of liquid boer goat semen in tris egg yolk extender enriched with non-enzymatic antioxidants. J. Kedokteran Hewan

[24] Zahra, N. N., Amelinda, N., Hanun, N. Z., Yulistiani, F. & Heriyanto. 2021. Studi Literatur Implementasi Metode Microwave Assisted Extraction (MAE) Untuk Ekstraksi Fenol Dengan Pelarut Etanol. Fluida, 14(2): 80–87. https://doi.org/10.35313/fluida.v14i2.2542

Downloads

Download data is not yet available.