Physicochemical Characteristics of Artificial Nori Made from Water Clover (Marsilea crenata) Leaves with Different Concentrations of Carrageenan

Devianra Luthfi Alamsyah (1), Dedin Finatsiyatull Rosida (2), Yunita Satya Pratiwi (3)
(1) Food Technology Department, Faculty of Engineering and Science, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya, Indonesia
(2) Food Technology Department, Faculty of Engineering and Science, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya, Indonesia and Innovation Center of Appropriate Food Technology for Lowland and Coastal Area, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Indonesia
(3) Food Technology Department, Faculty of Engineering and Science, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya, Indonesia
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How to cite (AJARCDE) :
Alamsyah, D. L., Rosida, D. F., & Pratiwi, Y. S. (2026). Physicochemical Characteristics of Artificial Nori Made from Water Clover (Marsilea crenata) Leaves with Different Concentrations of Carrageenan. AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment), 10(3), 627–632. https://doi.org/10.29165/ajarcde.v10i3.1310

Indonesia relies heavily on importing commercial seaweed nori, reaching 321 tons annually. To reduce import dependency and utilize local botanical resources, water clover (Marsilea crenata), an endemic bioactive-rich fern from East Java, represents a promising alternative raw material. This study aimed to characterize the physicochemical and mechanical properties of artificial nori made from water clover leaves with varying carrageenan concentrations (1%, 1.5%, and 2%). The resulting sheets were analyzed for yield, moisture, ash, protein, crude fiber, total phenolic content, antioxidant activity, tensile strength, and thickness. Results indicated that higher carrageenan concentrations increased yield, moisture, ash, crude fiber, thickness, and tensile strength due to the internal bonds between carrageenan. Conversely, total phenolic content and antioxidant activity decreased because bioactive compounds were less extractable due to entrapment within the matrix. This research requires further optimization to match the mechanical tensile strength of commercial seaweed-based nori.


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[1] R. R. A. Nurcahyani, S. Aminah, and M. F. Kurniawan, “Karakteristik organoleptik dan kimia snack nori dari daun chaya dan tapioka,” J. Pangan dan Gizi, vol. 12, no. 1, pp. 60–70, 2022, doi: 10.26714/jpg.12.1.2022.60-70.

[2] J. Cao, J. Wang, S. Wang, and X. Xu, “Porphyra species: A mini-review of its pharmacological and nutritional properties,” J. Med. Food, vol. 19, no. 2, pp. 111–119, 2016, doi: 10.1089/jmf.2015.3426.

[3] E. Sinurat, D. Fransiska, B. S. B. Utomo, Subaryono, Nurayati, and Sihono, “Characteristics of Nori-Like Product Prepared from Seaweeds Growing in Indonesia,” J. Aquat. Food Prod. Technol., vol. 31, no. 6, pp. 525–535, 2022, doi: https://doi.org/10.1080/10498850.2022.2077677.

[4] B. Ma’arif, M. Agil, and H. Laswati, “Phytochemical Assessment on n-Butanol ekstract and fractions of Marsilea crenata Presl. Leaves Through GC-MS,” J. Tradit. Med., vol. 21, no. 2, pp. 77–85, 2016.

[5] Wisanti, D. C. Aloysius, S. Zubaidah, and S. R. Lestari, “Morphological character variation of marsilea crenata L. Living in floating aquatic, emergent aquatic, and terrestrial,” Biodiversitas, vol. 22, no. 7, pp. 2853–2860, 2021, doi: 10.13057/biodiv/d220736.

[6] Y. Ermawati, A. Aminatuzzuhro, and S. M. Maulana, “Menggali Potensi Lokal melalui Pengembangan UMKM dan Peluang Desa Wisata dalam Pemulihan Covid-19 di Kampung Semanggi, Surabaya,” Fokus ABDIMAS, vol. 01, no. 01, pp. 18–24, 2022, [Online]. Available: https://www.ejournal.stiepena.ac.id/index.php/abdimas/article/view/525%0Ahttps://www.ejournal.stiepena.ac.id/index.php/abdimas/article/viewFile/525/357

[7] Nurjanah, A. Azka, and A. Abdullah, “Aktivitas Antioksidan Dan Komponen Bioaktif Semanggi Air (Marsilea Crenata),” J. Inov. dan Kewirausahaan, vol. 1, no. 3, pp. 152–158, 2012.

[8] S. Fatimah, E. Suprasetya, and J. Y. Hernawan, “Aktivitas Antiinflamasi Ekstrak Etanol Daun SEmanggi Air (Marsilea crenata) pada Mencit Putih (Mus musculus L.) dengan Induksi Karagenin,” J. Permata Indones., vol. 15, no. 1, pp. 48–50, 2024.

[9] E. Desiana and T. Y. Hendrawati, “Pembuatan karagenan dari Eucheuma cottoni dengan ekstraksi KOH menggunakan variabel waktu ekstraksi,” in Seminar Nasional Sains dan Teknologi, 2015, pp. 1–7.

[10] W. Atmaka, Af’idatusholikhah, S. Prabawa, and B. Yudhistira, “Pengaruh variasi konsentrasi kappa karagenan terhadap Karakteristik fisik dan kimia gel cincau hijau (Cyclea barbata L. Miers),” War. Ind. Has. Pertan., vol. 38, no. 1, pp. 25–35, 2021, doi: 10.32765/wartaihp.v38i1.6093.

[11] S. D. Prabaningrum, V. P. Bintoro, and S. B. M. Abduh, “Pengaruh konsentrasi bahan pengikat terhadap nilai rendemen, kadar air, aktivitas air, dan warna pada nori artifisial daun cincau,” J. Apl. Teknol. Pangan, vol. 11, no. 2, pp. 47–52, 2022, [Online]. Available: https://doi.org/10.17728/jatp.14367

[12] Sihono et al., “Optimization of Nori-Like Product Formulation from Ulva spp., Gracilaria sp., and Glycerol Using Mixture Design Method,” J. Pengolah. Has. Perikan. Indones., vol. 26, no. 3, pp. 433–447, 2023, doi: 10.17844/jphpi.v26i3.48337.

[13] J. Zhang, T. Nagahama, H. Ohwaki, Y. Ishibashi, Y. Fujita, and S. Yamazaki, “Analytical Approach to the Discoloration of Edible Laver ‘Nori’ in the Ariake Sea,” Anal. Sci., vol. 20, no. 1, pp. 37–43, 2004, doi: 10.2116/analsci.20.37.

[14] S. F. Isnaini, “Karakteristik Nori Dari Daun Kelor Dengan Penambahan Karagenan Dan Pati Garut Sebagai Bahan Pembentuk Gel,” Universitas Jember, 2018. [Online]. Available: https://repository.unej.ac.id/xmlui/bitstream/handle/123456789/100892/Syayyidah Faizatul Isnaini - 141710101069.pdf?sequence=1

[15] N. Stevani, A. Mustofa, and Y. W. Wulandari, “Pengaruh lama pengeringan dan penambahan karagenan terhadap karakteristik nori daun kangkung (Ipomoea reptans Poir),” J. Teknol. dan Ind. Pangan, vol. 3, no. 2, pp. 85–96, 2019.

[16] E. Sinurat, Nurhayati, D. Fransiska, and Sihono, “Substitution of red seaweed (Porphyra) with other seaweeds in nori making,” IOP Conf. Ser. Earth Environ. Sci., vol. 733, no. 1, 2021, doi: 10.1088/1755-1315/733/1/012109.

[17] M. Agil, I. Kusumawati, and N. Purwitasari, “Phenotypic variation profile of Marsilea crenata Presl. cultivated in water and in the soil,” J. Bot., vol. 2017, 2017, doi: 10.1155/2017/7232171.

[18] C. Popescu, M. Iordan, and B. Cristian, “Structure and properties of carrageenan,” Polym Chem, pp. 27–32, 2007.

[19] C. Anwar, I. Irmayanti, and G. Ambartiasari, “Pengaruh Lama Pengeringan terhadap Rendemen , Kadar Air , dan Organoleptik Dendeng Sayat Daging Ayam,” J. Peternak. Sriwij., vol. 10, no. 2, pp. 29–38, 2021.

[20] A. Célino, S. Fréour, F. Jacquemin, and P. Casari, “The hygroscopic behavior of plant fibers?: a review,” Front. Chem., vol. 1, no. 43, pp. 1–12, 2014, doi: 10.3389/fchem.2013.00043.

[21] T. G. M. Van De Ven, Cellulose-Fundamental Aspects. Intech, 2013.

[22] S. Sabil, F. Maruddin, T. Wahyuni, and M. Taufik, “Edible film characteristics at different casein concentrations,” in IOP Conference Series: Earth and Environmental Science, 2021, pp. 1–7. doi: 10.1088/1755-1315/788/1/012115.

[23] D. F. Rosida, F. Putri Nurani, and M. I. M. Danil Ilmi, “The Nori Gracilaria sp. with a Variation of Stabilizers as Healthy Food,” IOP Conf. Ser. Mater. Sci. Eng., vol. 1125, no. 1, p. 012104, 2021, doi: 10.1088/1757-899x/1125/1/012104.

[24] J. F. Kurnia, E. N. Dewi, and R. A. Kurniasih, “Pengaruh konsentrasi bubur Eucheuma cottonii terhadap karakteristik selai lembaran,” J. ilmu dan Teknol. Perikan., vol. 3, no. 1, pp. 43–49, 2021.

[25] M. Limonu, Y. Bait, A. Engelen, and N. Muhsin, “Seaweed nori (Kappapycus alvarezi) physicochemical and organoleptic characteristics with moringa leaf fortification (Moringa oleifera LAM),” Agrointek J. Teknol. Ind. Pertan., vol. 18, no. 2, pp. 360–372, 2024, doi: 10.21107/agrointek.v18i2.16764.

[26] D. Handito, “Pengaruh konsentrasi karagenan terhadap sifat fisik dan mekanik edible film,” Agroteksos, vol. 21, no. 2, pp. 151–157, 2011.

[27] S. Wahyuni, Holilah, Asranudin, M. I. K. Rianse, and M. S. Sadimantara, “Effect of ?-carrageenan concentration on physical and Mechanical properties of vegetable leather based on kelor leaves (Moringa oleifera L.),” in IOP Conference Series: Earth and Environmental Science, 2019, vol. 260. doi: 10.1088/1755-1315/260/1/012180.

[28] M. S. Siregar, I. Syukri, H. Rusmarilin, and D. Ardilla, “Studi Pembuatan Minuman Serat Alami yang Kaya ?-Karoten,” J. Teknol. dan Ind. Pertan. Indones., vol. 15, no. 01, pp. 8–15, 2023.

[29] A. Wulansari, R. Andriani, and E. K. Dewi, “Variasi Bahan Baku dan Metode Pembuatan Nori Tiruan: Kajian Pustaka,” vol. 3, no. 1, pp. 1–11, 2020.

[30] D. Fransiska et al., “Karakteristik Nori Campuran Rumput Laut Ulva sp. dan Gracilaria sp. yang Diproses dengan Metode Casting,” J. Pascapanen dan Bioteknol. Kelaut. dan Perikan., vol. 17, no. 2, p. 99, 2022, doi: 10.15578/jpbkp.v17i2.728.

[31] A. M. Jacoeb, Nurjanah, M. Arifin, W. Sulistiono, and S. S. Kristiono, “Deskripsi Histologis dan Perubahan Komposisi Kimia Daun dan Tangkai Semanggi Air (Marsilea crenata, Presl., Marsileaccae) Akibat Perebusan,” Jurnal Pengolahan Hasil Perikanan Indonesia, vol. 13, no. 2. pp. 81–95, 2010.

[32] S. A. P. Simangungsong and Rosida, “The Effect of Carrageenan and Glycerol Addition on the Physicochemical Characteristics of Nori, an Analogue of Genjer Leaves (Limnocharis flava),” Asian J. Appl. Res. Community Dev. Empower., vol. 10, no. 1, pp. 174–178, 2026.

[33] P. P. Pamungkas, S. S. Yuwono, and K. Fibrianto, “Potensi rumput laut merah (Gracilaria gigas) dan penambahan daun kenikir (Cosmos caudatus) sebagai bahan baku pembuatan nori,” J. Teknol. Pertan., vol. 20, no. 3, pp. 171–180, 2019.

[34] F. Rachmadiarti and G. Trimulyono, “Phytoremediation capability of water clover (Marsilea crenata (L). Presl.) in synthetic Pb solution,” Appl. Ecol. Environ. Res., vol. 17, no. 4, pp. 9609–9619, 2019.

[35] O. N. Makshakova and Y. F. Zuev, “Interaction-Induced Structural Transformations in Polysaccharide and Protein-Polysaccharide Gels as Functional Basis for Novel Soft-Matter?: A Case of Carrageenans,” Gels, vol. 8, no. 287, pp. 1–17, 2022.

[36] P. S. Chauhan and A. Saxena, “Bacterial carrageenases: an overview of production and biotechnological applications,” 3 Biotech, vol. 6, no. 146, pp. 1–18, 2016, doi: 10.1007/s13205-016-0461-3.

[37] C. Madhu, B. Sanjay, K. Harshitha, J. Pradham, and S. S. N. Sree, “Review article on crude fiber,” J. Multidiscip. Res., vol. 5, no. 3, pp. 30–33, 2025.

[38] S. Naumann, U. Schweiggert-weisz, A. Martin, M. Schuster, and P. Eisner, “Food Hydrocolloids Effects of extrusion processing on the physiochemical and functional properties of lupin kernel fibre,” Food Hydrocoll., vol. 111, pp. 1–11, 2021, doi: 10.1016/j.foodhyd.2020.106222.

[39] S. N. Anggraeni, “Karakteristik Vegetable Leather Daun Alur (Suaeda maritima) dengan Variasi Konsentrasi Pektin dan Sukrosa,” Universitas Pembangunan “Veteran” Nasional Jawa Timur, 2025.

[40] L. Jakobek, “Interactions of polyphenols with carbohydrates , lipids and proteins,” Food Chem., vol. 175, pp. 556–567, 2015, doi: 10.1016/j.foodchem.2014.12.013.

[41] R. R. Kotha, F. S. Tareq, E. Yildiz, and D. L. Luthria, “Oxidative Stress and Antioxidants—A Critical Review on In Vitro Antioxidant Assays,” Antioxidants, vol. 11, no. 2388, 2022.

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