DOI : https://doi.org/10.29165/ajarcde.v10i3.1179

Qualitative Phytochemical Screening of Cladophora glomerata Macroalgae Extract Obtained Using Microwave-Assisted Extraction (MAE)

Mufida Naima Rohmah (1), Dedin Finatsiyatul Rosida (2), Riski Ayu Anggreini (3)
(1) Food Technology Department, Faculty of Engineering and Science, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Indonesia
(2) Food Technology Department, Faculty of Engineering and Science, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Indonesia and Innovation Center of Appropriate Food Technology for Lowland and Coastal Area, Universitas Pembangunan Nasional Veteran Jawa Timur, Indonesia.
(3) Food Technology Department, Faculty of Engineering and Science, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Indonesia
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Rohmah, M. N., Rosida, D. F., & Anggreini, R. A. (2026). Qualitative Phytochemical Screening of Cladophora glomerata Macroalgae Extract Obtained Using Microwave-Assisted Extraction (MAE). AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment), 10(3), 620–626. https://doi.org/10.29165/ajarcde.v10i3.1179
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This study aimed to qualitatively identify classes of secondary metabolites in Cladophora glomerata extract obtained via microwave-assisted extraction (MAE) using distilled water as the solvent for 5 minutes. We used a 3 × 3 factorial design with three replicates, resulting in nine treatment combinations. Treatments included solid-to-solvent ratios of 1:30, 1:40, and 1:50 g/mL and microwave power levels of 300, 450, and 600 watts. We conducted qualitative phytochemical screening for six classes of secondary metabolites: phenolics, tannins, flavonoids, alkaloids, saponins, and triterpenoids. The results showed that phenolics, tannins, and saponins were qualitatively detected in all extracts across all treatment combinations. Conversely, flavonoids, alkaloids, and triterpenoids were not detected in any tested extraction combination. These findings provide preliminary qualitative evidence of phenolics, tannins, and saponins in Cladophora glomerata extract obtained via MAE using distilled water.


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Buschmann, A. H., Camus, C., Infante, J., Neori, A., Israel, Á., Hernández-González, M. C., Pereda, S. V., Gomez-Pinchetti, J. L., Golberg, A., Tadmor-Shalev, N., & et al. (2017). Seaweed Production: Overview of the Global State of Exploitation, Farming, and Emerging Research Activity. Eur. J. Phycol, 52, 391-406.

Michalak, I., & Messyasz, B. (2021). Concise Review of Cladophora spp.: Macroalgae of Commercial Interest. Journal of Applied Phycology, 33, 133-166.

Nami, N., Ebadi, S. F., Otaghsara, S. F. T., Baei, M., & Rahimi, E. S. (2016). GC-MS Analysis and Preliminary Test of Phytochemical Screening of Crude Ethanolic Extract of Green Algae, Cladophora glomerata (L.) Kütz from Caspian Sea. Iranian Journal of Organic Chemistry, 8(4), 1899-1907.

Fabrowska, J., ??ska, B., & Schroeder, G. (2015). Freshwater Cladophora glomerata as a New Potential Cosmetic Raw Material. Chemik, 69(8), 491-497.

Korzeniowska, K., Leska, B., & Wieczorek, P. P. (2020). Isolation and Determination of Phenolic Compounds from Freshwater Cladophora glomerata. Algal Res, 48, 101912.

Munir, M., Qureshi, R., Bibi, M., & Khan, A. M. (2019). Pharmaceutical Aptitude of Cladophora: A Comprehensive Review. Algal Res, 39, 101476.

Vuong, D., Kaplan, M., Lacey, H. J., Crombie, A., Lacey, E., & Piggott, A. M. 2018. A Study of the Chemical Diversity of Macroalgae from South Eastern Australia. Fitoterapia, 126, 53-64.

Butar-Butar, R. G. S., Neswita, E., Sembiring, N. B., Novriani, E., Simanjuntak, N. J. P., & Pakpahan, E. H. 2023. Phytochemical screening test and measurement of total flavonoid levels in paku (Nephrolepis biserrata) extract with n-hexane, ethyl acetate, and water fractions. Journal of Pharmaceutical and Sciences, 6(3), 1142-1160.

Fabrowska, J., Messyasz, B., Szyling, J., Walkowiak, J., & ?eska, B. (2018). Isolation of Chlorophylls and Carotenoids from Freshwater Algae Using Different Extraction Methods. Phycol. Res, 66, 52-57.

Vázquez-Delfín, E., Robledo, D., & Freile-Pelegrín, Y. (2014). Microwave Assisted Extraction of the Carrageenan from Hypnea musciformis (Cystocloniaceae, Rhodophyta). J Appl Phycol, 26, 901-907.

Jain, T., Jain, V., Pandey, R., Vyas, A., & Shukla, S. (2009). Microwave Assisted Extraction for Phytoconstituents-an overview. Asian J Res Chem, 2, 19-25.

Khuantrairong. T., & Traichaiyaporn. S. (2011). The Nutritional Value of Edible Freshwater Alga Cladophora sp. (Chlorophyta) Grown Under Different Phosphorus Concentrations. Int J Agric Biol. 13, 297-300.

Michalak, I., Mironiuk, M., & Marycz, K. (2018). A Comprehensive Analysis of Biosorption of Metal Ions by Macroalgae Using ICP-OES, SEM-EDX, and FTIR Techniques. PLoS ONE, 13, e0205590.

Fahrurrozi, L. A. (2021). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Petai Cina (Leucaena glauca (L.) Benth.) dengan Metode DPPH (2,2-diphenyl-1 picrylhydrazyl). Sinteza, 1(1), 27-32.

Sabaragamuwa, R., & Perera, C. O. 2023. Total Triterpenes, Polyphenols, Flavonoids, and Antioxidant Activity of Bioactive Phytochemicals of Centella asiatica by Different Extraction Techniques. Foods, 12, 3972.

Rostagno, M. A., D’Arrigo, M., Martínez, J. A., & Martínez, J. A., (2010). Combinatory and Hyphenated Sample Preparation for the Determination of Bioactive Compounds in Foods. Trends Analy. Chem. 29, 553–561.

Esquivel?Hernández, D. A., Ibarra?Garza, I. P., Rodríguez?Rodríguez, J., Cuéllar?Bermúdez, S. P., Rostro?Alanis, M. D. J., Alemán?Nava, G. S., García?Pérez, J. S., & Parra?Saldívar, R. (2017). Green Extraction Technologies For High?Value Metabolites from Algae: A Review. Biofuels Bioprod. Biorefin, 11, 215-231.

Wadli, W., & Hasdar, M. (2021). Ekstraksi Beras Hitam Sirampog Berbantu Gelombang Mikro (Microwave Assisted Extraction) (MAE)). Jurnal Pengolahan Pangan, 6(2), 49-53.

Mirzadeh, M., Arianejad, M. R., & Khedmat, L. (2020). Antioxidant, Antiradical, and Antimicrobial Activities of Polysaccharides Obtained by Microwave Assisted Extraction Method: A Review. Carbohydr. Polym, 229, 115421.

Mandal, V., & Mandal, S. C. (2010). Design and Performance Evaluation of a Microwave Based Low Carbon Yielding Extraction Technique for Naturally Occurring Bioactive Triterpenoid: Oleanolic Acid. Biochem. Eng. J. 50, 63-70.

AOAC. 2005. Official Method of Analysis. AOAC International, Arlington.

Sari, B. L., Triastinurmiatiningsih, & Haryani, T. S. Optimasi metode Microwave-Assisted Extraction (MAE) untuk Menentukan Kadar Flavonoid Total Alga Coklat Padina australis. Alchemy: Jurnal Penelitian Kimia, 16(1), 38–49.

Harborne, J. B. (1987). Metode fitokimia: Penuntun cara modern menganalisis tumbuhan (Kosasih, P., dan Iwang, S. J., Penerjemah). Bandung, Indonesia: ITB.

Sulasmi, E. S., Wuriana, Z. F., Sari, M. S., & Suhadi, S. (2018). Analisis Kualitatif Kandungan Senyawa Aktif (Flavonoid, Alkaloid, Polifenol, Saponin, Terpenoid, dan Tanin) pada Ekstrak Metanol Daun dan Rhizoma Phymatodes scolopendria (Burm) Ching di Taman Nasional Baluran. In Prosiding Seminar Nasional Hayati. 6, 121-128.

Sirbu, R., Negreanu-Pirjol, T., Mirea, M., & Negreanu-Pirjol, B. S. (2020). Bioactive Compounds from Three Green Algae Species Along Romanian Black Sea Coast with Therapeutically Properties. European Journal of Natural Sciences and Medicine, 3(1), 87-106.

Yarnpakdee, S., Benjakul, S., & Senphan, T. (2018). Antioxidant Activity of the Extracts from Freshwater Macroalgae (Cladophora glomerata) Grown in Northern Thailand and its Preventive Effect Against Lipid Oxidation of Refrigerated Eastern Little Tuna Slice. Turkish J. Fish. Aquat. Sci, 19, 209-219.

Sari, D. K., Kustiningsih, I., & Lestari, R. S. D. (2017). Pengaruh Suhu dan Waktu Pengeringan terhadap Mutu Rumput Laut Kering. Teknika: Jurnal Sains dan Teknologi, 13(1), 43-50.

Lovric, V., Putnik, P., Bursac Kovacevic, D., Jukic, M., & Dragovic-Uzalec, V. (2017). The Effect of Microwave Assisted Extraction on the Phenolic Compounds and Antioxidant Capacity of Blackthorn Flowers. Food Technology and Biotechnology, 55(2), 243-250.

Xiao, W., Han, L., & Shi, B. (2008). Microwave-Assisted Extraction of Flavonoids from Radix astragali. Sep. Purif. Technol, 62, 614-618.

Llompart, M., Garcia-Jares, C., Celeiro, M., & Dagnac, T. (2019). Extraction | Microwave-Assisted Extraction, 3rd ed., Elsevier Inc, 67-77.

Ciuperca, O. T., Ionescu, E., Secula, M. S., & Volf, I. (2023). Microwave Assisted Extraction of Condensed Tannins from Branches of Prunus spinose L.: Response Surface Modeling and Optimization. Processes, 11(7), 2024.

Liang, Q., Chen, H., Zhou, X., Deng, Q., Hu, E., Zhao, C., et al. (2017). Optimized Microwave-Assistant Extraction Combined Ultrasonic Pretreatment of Flavonoids from Periploca forrestii Schltr and Evaluation of its Anti-Allergic Activity. Electrophoresis, 38, 1113–1121.

Proestos, C., & Komaitis, M. (2008). Application of Microwave-Assisted Extraction for Rapid Extraction of Plant Phenolic Compounds. LWT-Food Science and Technology, 41(4), 652-659.

Abdullahi, M., Ibrahim, M. A., & Usman, H. (2016). Phytochemical Screening and Antimicrobial Activity of the Leaf Extract of Ceropegia variegata (Linn.) Wight and Arn. African Journal of Pharmacy and Pharmacology, 10(2), 18–23.

Putri, N. M., Wiraningtyas, A., & Mutmainah, P. A. (2021). Perbandingan Metode Ekstraksi Senyawa Aktif Daun Kelor (Moringa oleifera): Metode Maserasi dan Microwave-Assisted Extraction (MAE). Dalton: Jurnal Pendidikan Kimia Dan Ilmu Kimia, 4(2), 25–33.

Tan, S. P., Vuong, Q. V., Stathopoulos, C. E., Parks, S. E., & Roach, P. D. (2014). Optimized Aqueous Extraction of Saponins from Bitter Melon for Production of a Saponin-Enriched Bitter Melon Powder. Journal of Food Science, 79, E1372–1381.

Amarni, F., & Kadi, H. (2010). Microwave-Assisted Solvent Kinetics Study Oil Extraction from Olive Cake using Hexane: Comparison with Conventional Extraction. Innovative Food Science and New Technologies, 11(2), 322-327.

Nahar, L. (2012). Em Natural Products Isolation; Sarker, S. D., Nahar, L., eds. Humana Press: Totowa. 43-74.

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